Part:BBa_M36596:Experience

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Applications of BBa_M36596

This part is designed to create hydrogenase enzymes that can produce hydrogen within organisms such as E. Coli and S. Elongatus and are not inactivated by the presence of oxygen. The success of this goal would allow a more efficient method of biohydrogen production on an industrial scale. In our experiments, this part was used in conjunction with a rhamnose sensor, as well as a kanamyacin selectable marker and origin of replication (high). It was transfected successfully into E. Coli and unsuccessfully into S. Elongatus.

Sources of subparts

Bicistronic RBS: Mutalik VK, Guimaraes JC, Cambray G, Mai QA, Christoffersen MJ, Martin L, Yua A, Lam C, Rodriguez C, Bennett G, et al. Quantitative estimation of activity and quality for collections of functional genetic elements. Nat. Methods. 2013;10:347-353. BioFAB. (http://io.biofab.org:5000/simple/randomized_bds)

Sequence for large subunit Hydrogenase: Nishihara H, Miyata Y, Miyashita Y, Bernhard M, Pohlmann A, et al. Analysis of the molecular species of hydrogenase in the cells of an obligately chemolithoautotrophic, marine hydrogen-oxidizing bacterium, Hydrogenovibrio marinus. Biosci Biotechnol Biochem. 2001;65:2780–2784. UnitPRO (http://www.uniprot.org/uniprot/F2Z6J6).

High efficiency transcriptional terminator: Sunil Bodapati, Julia Joung, Daniel Esquivel-Reynoso. Part: BBa_M36281: Hard Information. Registry of Standard Biological Parts. (http://partsregistry.org/cgi/partsdb/part_info.cgi?part_name=BBa%20M36281)

User Reviews

UNIQ74f0f82472087486-partinfo-00000000-QINU UNIQ74f0f82472087486-partinfo-00000001-QINU